Fig. 9: Fluorescence compensation standards with dye-conjugated antibodies, anti-CD3 V500, anti-CD14 V450, and anti-CD19 PE.
From: Single drop cytometry onboard the International Space Station
a Left-to-right: ground preflight benchmark cytometer data showing scatterplots for blue–green, orange–green, orange–blue, and a log10 histogram of orange FL2 burst intensities. b Corresponding rHEALTH ONE preflight ground sample analysis. A transparent color overlay is included to highlight the quadrants that are unlabeled (i.e., BL−, GR−), labeled with a single color (i.e., GR+, BL−), or labeled with both colors (i.e., GR+, BL+). The color channel abbreviations are green (GR), blue (BL), and orange (OR). c Corresponding rHEALTH ONE on-orbit flight data. d Ground postflight benchmark flow cytometer data. The quadrant analysis is moved to match the percentage of beads seen in the preflight analysis. Source data filenames are provided in the Source Data File.
