Fig. 3: Cross-species transfer of 2,3-butanediol (2,3-BDO) utilization iModulon of Pseudomonas putida in E. coli.

A A pathway responsible for 2,3-BDO utilization. B Scatter plot shows weights of genes in P. putida to AcoR iModulon. Gray lines indicate thresholds for determining iModulon membership. Five genes constitute the AcoR iModulon (orange circles). Gray circles identify genes not in the iModulon. Black circles are three neighboring genes. C Genomic structure of the AcoR iModulon. Orange shade shows predicted operonic structure. Genes in the iModulon are in orange. Arrows indicate three different plasmid constructs for cross-species transfer. D 2,3-BDO degradation by P. putida. The formation of acetoin was negligible. Blue and yellow boxes represent 2,3-BDO and acetoin in the culture medium. Red circles show cell density. Dots indicate individual data points. Data were presented as mean values ± SD. Error bars indicate the SD of the three biological replicates. E 2,3-BDO and acetoin degradation by E. coli carrying empty plasmid or one of the three constructs. 2,3-BDO was added at the start of the culture and the remaining amount and acetoin formation was measured. Blue and yellow boxes represent 2,3-BDO and acetoin in the culture medium. Red circles show cell density. Dots indicate individual data points. Data were presented as mean values ± SD. Error bars indicate SD of the three biological replicates. Source data are provided as a Source Data file.