Fig. 4: The ATPase activity of SWI/SNF complex is required for POU2AF2 mediated transcriptional regulation.

a The scatter plot shows the correlation of gene expression change when POU2AF2 were depleted by sgRNAs or cells were treated with BRM014. The significantly altered genes (|log2FC|>1, adj. p < 0.01) were highlighted in red (upregulated, n = 239) or blue (downregulated, n = 274). Data are derived from two biological replicates. Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p-values less than 0.01 were considered to be differentially expressed in the EdgeR analysis⁴⁸. b The log2 fold change heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in BRM014 (1 μM) treated NCI-H526 cells. Data are derived from two biological replicates. c The ATAC-seq experiment was conducted in NCI-H526 cells treated with either BRM014 or DMSO. The average plot shows the ATAC-seq signal centered at the three clusters. d The heatmap bar plot shows H3K4me1 and H3K27ac levels at Cluster 2 (active enhancer) peaks between DMSO and BRM014 treated cells. e The NCI-H526 cells were treated with either DMSO or BRM014 (1 μM) for 24 hours. The mRNA levels of TAS1R3, IRAG2, AVIL, CHAT, GNG13, and NREP were determined by real time PCR. n = 3 technical replicates. Data are presented as mean values ± standard deviation (SD). Source data are provided as a Source Data file. f The track example shows the H3K27ac, H3K4me1, and ATAC-seq signals in cells treated with either DMSO or BRM014 at active enhancer locus of CHAT gene. g The average plot shows the chromatin occupancy of EZH2 and H3K27me3 levels at Cluster 3 in NCI-H526 cells treated with either DMSO or BRM014. h The track example shows the ATAC-seq signals, H3K27me3 levels and EZH2 occupancy at EYA1 gene locus in NCI-H526 cells treated with either DMSO or BRM014. i NCI-H526 cells were treated with 1 μM BRM014 for 24 hours. The mRNA levels of FOXO1, IGFBP2, UBASH3B, and EYA1 were determined by real time PCR. n = 3 technical replicates. Data are presented as mean values ± standard deviation (SD). Source data are provided as a Source Data file.