Fig. 2: SET-26 and HCF-1 regulate a common set of genes.

Venn diagrams showing genes (a) upregulated or (b) downregulated in RNA expression in germline-less day 1 adult glp-1(-);set-26(-) or glp-1(-);hcf-1(-) double mutants compared to glp-1(-) single mutants and the overlapping 485 upregulated, or 602 downregulated genes with significant expression changes in both mutants. c Heatmap showing hierarchical clustering of all differentially expressed genes in either glp-1(-);set-26(-) or glp-1(-);hcf-1(-) double mutants compared to glp-1(-) single mutants; The log2 fold change of RNA expression in each double mutant versus control was used for the clustering analysis and is shown as indicated on the scale. d Venn diagram showing the genes bound by SET-26 and HCF-1 as determined in Fig. 1h and the genes commonly upregulated or downregulated in RNA expression when set-26 or hcf-1 is inactivated as determined in (a, b). 445 genes are bound by both SET-26 and HCF-1 and show significant RNA expression change in both mutants. GO term analysis by Wormcat of SET-26 and HCF-1 direct somatic targets that are (e) upregulated (206) or (f) downregulated (239) in RNA expression in (d). In (a) and (b), *** indicates p < 1 × 10⁻¹⁵ and the overlap is higher than expected by chance, as calculated by one-sided Fisher’s Test. In (e) and (f), Wormcat p values are determined by one-sided Fisher test with FDR correction. Gene sets are provided in Supplementary Data 3. N = 2 for RNA-seq.