Fig. 3: HCF-1 recruitment to chromatin in somatic cells requires SET-26.

a Screenshot from IGV shows normalized somatic HCF-1 binding and peak calls in a portion of Chromosome V (captured by CUT&RUN of hcf-1::gfp::3xflag worms, N = 2) in control worms or set-26(−) mutants grown on glp-1 RNAi. b Number of HCF-1 peaks called in combined replicates of controls or set-26(−) mutants grown on glp-1 RNAi. c Metaplot (top) and heatmap (bottom) of z-scores representing normalized HCF-1 signal in somatic HCF-1 binding sites and surrounding 2 kb up- and downstream in either controls or set-26(−) mutants grown on glp-1 RNAi. d Volcano plot of HCF-1 binding regions determined by DiffBind to be significantly different (pink, FDR < = 0.05) or unchanged (blue, FDR > 0.05) in set-26(−) mutants compared to controls grown on glp-1 RNAi. DiffBind FDR values are calculated using DESeq2. e Venn diagram of genes with significantly lower HCF-1 binding in set-26(-) mutants grown on glp-1 RNAi (as determined in d) and the overlap with genes up- or downregulated in RNA expression in germline-less set-26(-) and hcf-1(-) mutants (from Fig. 2a, b). Wormcat GO enrichment analysis for genes with lower HCF-1 binding in set-26(-) mutants on glp-1 RNAi that are either (f) upregulated or (g) downregulated in RNA expression in both set-26(-) and hcf-1(-) germline-less mutants (from e). Wormcat p values are determined by one-sided Fisher test with FDR correction. Gene sets and differential peaks are provided in Supplementary Data 3 and 4.