Fig. 5: HDA-1 is required for the full longevity of set-26(-) and hcf-1(-) mutants and co-occupies many binding sites with SET-26 and HCF-1. | Nature Communications

Fig. 5: HDA-1 is required for the full longevity of set-26(-) and hcf-1(-) mutants and co-occupies many binding sites with SET-26 and HCF-1.

From: The chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in C. elegans

Fig. 5

a Survival curves for wildtype controls, set-26(-), and hcf-1(-) mutants on E.V. control RNAi and hda-1 RNAi from one representative experiment (n = 108, 102, 107, 105, 104, and 106 worms, respectively). b Survival curves for glp-1(-) germline-less controls, glp-1(-);set-26(-), and glp-1(-);hcf-1(-) mutants on E.V. control RNAi and hda-1 RNAi from one representative experiment (n = 104, 104, 106, 104, 106, and 105 worms, respectively). hda-1 RNAi was initiated on day 1 of adulthood to avoid developmental defects caused by initiating hda-1 RNAi from egglay. N = 2 biological replicates for lifespan experiments. c Distribution of somatic HDA-1 peaks relative to the transcription start sites (TSS) of associated genes within 3 kb. Count represents the number of somatic peaks within each bin. Data were obtained from CUT&RUN assays in the glp-1(-);hda-1::gfp::ha tagged strain, N = 2. Screenshots from IGV show normalized somatic SET-26, HCF-1 (as in Fig. 1), and HDA-1 binding either in a (d) whole genome view, or a (e) close-up of a section of Chromosome II, with peaks called by MACS2 underneath the signal track for each factor. f Venn diagram showing somatic SET-26, HCF-1, and HDA-1 peaks in the glp-1(−) mutant background and 6082 peaks that overlap by 1 bp or more. In (f), *** indicates p < 1 × 10−15 and the overlap is higher than expected by chance, as calculated by one-sided hypergeometric test. Source data are provided as a Source Data file. Gene sets are provided in Supplementary Data 2.

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