Fig. 4: AFMFlip for serum-resistant membrane-fusion-mediated delivery.
From: An antifouling membrane-fusogenic liposome for effective intracellular delivery in vivo
a Uptake inhibition assay of AFMFlipYOYO-1 with the medium containing 0-50% FBS. The critical protein-resistance concentration was set as the FBS concentration with a significant change in the transportation mechanism. Data are presented as mean ± SD (n = 3 biologically independent samples). b CLSM observation of HeLa cells co-incubated with AFMFlipYOYO-1 in the medium containing 0-50% FBS for 2 h, showing their intracellular trafficking process. The nuclei were stained with DAPI, and the cellular lysosomes were labeled with LysoTracker. The experiment was repeated three times independently with similar results. c Pearson’s coefficient index of the fluorescence signals between green and magenta based on b was determined by ImageJ software. Data are presented as mean ± SD and statistically analyzed using one-way ANOVA (n = 3 biologically independent samples). ns: no significance (p > 0.05). d MFI of cells incubated with AFMFlipYOYO-1 in the medium containing FBS at the concentration from 0% to 50%. Data are presented as mean ± SD and statistically analyzed using one-way ANOVA (n = 3 biologically independent samples). ns: no significance (p > 0.05). e EGFP transfection efficiency of AFMFlippEGFP, MFlip-apEGFP, MFlip-bpEGFP, and Lipo2KpEGFP in the medium containing 0–50% FBS, analyzed via FACS. Data are presented as mean ± SD and statistically analyzed using one-way ANOVA (n = 3 biologically independent samples). ns: no significance (p > 0.05). f EGFP expression in HeLa cells observed by fluorescence microscopy transfected with AFMFlippEGFP at different FBS concentrations. The experiment was repeated three times independently with similar results. Source Data are provided as a Source Data file.
