Fig. 3: Comparison of BASALT with other binning tools for processing the CAMI-high dataset. | Nature Communications

Fig. 3: Comparison of BASALT with other binning tools for processing the CAMI-high dataset.

From: BASALT refines binning from metagenomic data and increases resolution of genome-resolved metagenomic analysis

Fig. 3

a Venn diagram showing the number of MAGs with quality ≥50 (completeness—5* contamination ≥ 50) recovered using BASALT (red), metaWRAP (cyan), DASTool (green) or VAMB (purple). b Completeness, contamination, and quality scores of shared 168 MAGs recovered using BASALT (B, red), DASTool (D, green), metaWRAP (M, cyan), or VAMB (V, purple). MAGs recovered using BASALT had significantly higher completeness (compared to VAMB, Tukey test, Benjamini–Hochberg adjusted P < 1 × 10−7) and significantly lower contamination (compared to all other tools, Tukey test, Benjamini–Hochberg adjusted P < 1 × 10−7), resulting in significantly higher quality scores compared to VAMB, DASTool and metaWRAP (Tukey test, Benjamini–Hochberg adjusted P < 1 × 10−7). c Pairwise comparison of MAGs shared between BASALT and VAMB (205 MAGs), BASALT and DASTool (210 MAGs), BASALT and metaWRAP (249 MAGs). BASALT obtained more MAGs with higher-quality scores than other tools. BASALT recovered more MAGs with higher quality (light green) than MAGs with lower (light red) or similar (difference ≤1, light blue) quality scores. d Number of MAGs recovered from CAMI-high dataset using DASTool (MaxBin2, CONCOCT, and MetaBAT2, MCM), VAMB, metaWRAP (MCM), or BASALT. Bar colors from light to dark indicate increasing MAG quality scores. The boxplot shows the distribution of data, the central dot in the box represents the median, the box bounds represent the 25th and 75th percentiles, and whiskers represent the minima to maxima values.

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