Fig. 2: Loss of YTHDF2 impairs myofiber growth irrespective of fiber-type distribution.
From: YTHDF2 governs muscle size through a targeted modulation of proteostasis
Representative wheat germ agglutinin (WGA, green) stained images for Ctrl and Y2-KO tibialis anterior at a 1 and b 2 months of age. Myofiber cross-sectional areas for c tibialis anterior and d quadriceps for Ctrl and Y2-KO mice at 1 and 2 months of age. e Fiber type distribution for Ctrl and Y2-KO tibialis anterior and f representative myosin type staining for type I (anti-BA-D5, red), type IIA (anti-SC-71, pink), type IIB (anti-BF-F3, pink), and all but IIX (anti-BF-35, pink) with counterstains anti-laminin (green) and DAPI (blue). Biological animal replicates n = 4 (Ctrl at 1 month), 4 (Y2-KO at 1 month), 7 (Ctrl at 2 months), and 6 (Y2-KO at 2 months) for panel c, d; n = 4 (Ctrl) and 4 (Y2-KO) for panel e. Data were presented as the mean ± SEM with the individual biological samples shown. Significance was determined by two-tailed Student’s t-test for comparisons between Ctrl and Y2-KO mice at 2 months, or by two-way ANOVA with Tukey’s HSD multiple-comparison test for comparison of the means of Ctrl and Y2-KO mice at 1 and 2 months of age. Welch’s correction was used for unequal variances: **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Scale bar = 125 µm for panel a, b. Scale bar = 100 µm for panel f.
