Fig. 4: YTHDF2-null muscles favor catabolic signaling.
From: YTHDF2 governs muscle size through a targeted modulation of proteostasis
a Western blot analyses and quantification using total protein detection by Ponceau stain as a loading control for b phosphor-(p-)AKT, c AKT, d p-AKT/AKT ratio, e p-mTOR, f mTOR, g p-mTOR/AKT ratio, h LC3I, i LC3II, j LC3II/LC3I ratio, k p62, l Beclin-1, m monoubiquitin, and n polyubiquitin in Ctrl and Y2-KO quadriceps at 8 months of age. o Proteasomal activity as determined via colorimetric proteasome activity assay kit in Ctrl and Y2-KO quadriceps. Biological animal replicates: n = 4 (Ctrl) and 4 (Y2-KO) in panel a–n; n = 3(Ctrl) and 3 (Y2-KO) in panel o. Data were presented as the mean ± SEM with the individual biological samples shown. Significance was determined by a two-tailed Student’s t-test for comparisons between Ctrl and Y2-KO mice. Welch’s correction was used for unequal variances: *p ≤ 0.05, **p ≤ 0.01.
