Fig. 7: HDACi improves the differentiation of human SF3B1^MUT erythroblasts.

a Scatter plot of fork speed measurement (kb/min) by DNA combing in 2 SF3B1^MUT (1 SF3B1/DNMT3A, 1 SF3B1/TET2/EP300) and 1 control ± HDACi 0.2 μM for 20 h expressed in means ± SD. Two-sided Mann-Whitney test. b Cell cycle analysis by BrdU incorporation in 6 SF3B1^MUT, 7 SF3B1^WT and 4 controls. Left panel: Mean percentages ± SD of S-phase cells. Right panel: Mean BrdU RFIs ± SD in S-phase. Two-sided paired t-test. SF3B1^MUT versus SF3B1^MUT + HDACi, P = 0.002. c–e Immunofluorescence images of p-RPA32s33 and γH2AX representative of 3 SF3B1^MUT, 6 SF3B1^WT MDS (1 SRSF2^MUT, 3 SF^WT, 2 w/o mutation) at day 11. Nuclei labelling with DAPI. Magnification X100 (scale: 20 µm). d, e Mean percentages ± SD of positive cells with > 5 intranuclear foci. f. Burst forming unit-erythroid (BFU-E) colony assays in 9 SF3B1^MUT, 8 SF3B1^WT and 5 controls. Mean ratios between HDACi and DMSO conditions ± SD. g May-Grünwald-Giemsa-stained cytospins (d12). h Proportions of erythroid precursors in 7 SF3B1^MUT, 3 SF3B1^WT and 4 controls at d7-10 and d14-16. Means ± SEM and 2-way ANOVA multiple comparisons for q values. i Scatter plots showing differentiation by flow cytometry expressed as mean percentages ± SD of GPA⁺CD49d^low cells. j Scatter plots showing mean percentages ± SD of dead cells (FSC/SSC) at d12-14. i, j Two-sided paired t-test for P values. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05. Source data are provided as a Source Data file.