Fig. 2: Characterization of resistant mutants.
From: Bacteria can compensate the fitness costs of amplified resistance genes via a bypass mechanism
A Changes in copy number of the resistance genes with increasing antibiotic concentration. B Relative growth rates with increasing antibiotic concentration. C Changes in the relative growth rate with increasing copy number of the resistance genes. Red represents the independent mutants isolated from DA33135 at each concentration of tobramycin tested (DA76789, DA76790, DA76791, DA76792 at 1X MIC; DA76805, DA76806, DA76807, DA76808 at 4X MIC; DA76821, DA76822, DA76823, DA76824 at 16X MIC; DA76837, DA76838, DA76839, DA76840 at 24X MIC), blue represents the independent mutants isolated from DA33137 at each concentration of gentamicin tested (DA76793, DA76794, DA76795, DA76796 at 1X MIC; DA76809, DA76810, DA76811, DA76812 at 4X MIC; DA76825, DA76826, DA76827, DA76828 at 16X MIC; DA76841, DA76842, DA76843, DA76844 at 24X MIC), green represents the independent mutants isolated from DA33140 at each concentration of gentamicin tested (DA76797, DA76798, DA76799, DA76800 at 1X MIC; DA76813, DA76814, DA76815, DA76816 at 4X MIC; DA76829, DA76830, DA76831, DA76832 at 16X MIC; DA76845, DA76846, DA76847, DA76848 at 24X MIC) and pink represents the independent mutants isolated from DA34827 at each concentration of tetracycline tested (DA76801, DA76802, DA76803, DA76804 at 1X MIC; DA76817, DA768118, DA76819, DA76820 at 4X MIC; DA76833, DA76834, DA76835, DA76836 at 16X MIC; DA76849, DA76850, DA76851, DA76852 at 24X MIC). Copy number was measured from the cell population for 3 independent lineages (biological replicates) and 1 technical replicate. Relative growth rate was measured from 6 biological replicates. Data are presented as mean values ± standard deviation indicated by error bars. Source data are provided as a Source Data file.
