Fig. 3: Relative growth rate and copy number of the compensated mutants and their respective parental strains.
From: Bacteria can compensate the fitness costs of amplified resistance genes via a bypass mechanism
A Compensated mutants of E. coli isolated on 24X MIC tobramycin after 100 generations (red). B Compensated mutants of E. coli isolated on 24X MIC gentamicin after 100 generations (blue). C Compensated mutants of K. pneumoniae isolated on 24X MIC gentamicin after 100 generations (green). D Compensated mutants of S. Typhimurium isolated on 24X MIC tetracycline after 100 generations (pink). The darker shade represents the high copy number parental strains isolated at 24X MIC and the lighter shades represent its three independent lineages for compensatory evolution experiment. Copy number (circles) was measured from the cell population for three independent lineages (biological replicates) and one technical replicate. Relative growth rate (box plots) was measured from six biological replicates. Data are presented as mean values ± standard deviation. The asterisks (*) indicate statistically significant changes in growth rate in the compensated mutants compared to their respective parental strains. The statistical testing was done using unpaired two-tailed Student’s t test. Exact P values are given in Source data file. For the box-plots, minima and maxima are the 25th and 75th percentiles respectively and the center is 50th percentile or the median value. The whiskers extend from the smallest to the largest value in the group. Source data are provided as a Source Data file.
