Fig. 2: Identification of OsMYB8 by comparative transcriptomic analysis of lodicules between indica and japonica.

a Principal component analysis (PCA) of the transcriptome datasets of lodicules between TFB and ZH11 at different time points. T18 and Z18 indicate 18:00 the day before floret opening; T9 and Z9 indicate 9:00 am (1 h and 3 h before floret opening in TFB and ZH11, respectively); Z11 indicate 11:00 am (1 h before opening in ZH11); TF and ZF indicate the time undergoing peak floret opening time (~10:00 am in TFB; ~12:00 noon in ZH11). b Venn Diagram showing the number of overlapping genes between the Cluster18 genes and up-regulated DEGs from T9 vs Z9. c Gene Ontology (GO) enrichment analysis of 251 overlap genes in (b). MF indicates molecular function, BP indicates biological process. The term of “transcription factor activity” is highlighted in red. d Expression analysis of 11 transcription factors selected through GO analysis in (c). The numbers in the heatmap represent the average FPKM values. e Reverse transcriptional quantitative PCR (RT-qPCR) analysis of OsMYB8 expression level in the lodicules of TFB and ZH11 at 9:00 am in June and October in 2021. Values are mean ± SEM. (n = 3 biological replicates). Significance is evaluated by the two-sided Student’s t-test at each time point, and P values are indicated. Source data are provided as a Source Data file.