Fig. 3: OsMYB8 positively regulates rice DFOT.

a Creation of the Osmyb8 mutants using the CRISPR/Cas9 genome editing approach. Osmyb8 mutants in ZH11 and TFB backgrounds were named as Osmyb8^ZH and Osmyb8^TF respectively. The upper panel shows a schematic diagram of the OsMYB8 gene bearing the CRISPR/Cas9 target site. The mutation site was indicated in red. b, e, j Comparison of panicles in ZH11 and Osmyb8^ZH mutants at 11:30 am (b) TFB and Osmyb8^TF mutants at 10:00 am (e) ZH11 and OsMYB8^TF/ZH11 lines at 10:00 am in June 2021 in Guangzhou (j). Scale bars, 1 cm. c, f, k Number of opened florets per panicle in ZH11 and Osmyb8^ZH mutants (c), TFB and Osmyb8^TF mutants (f), and ZH11 and OsMYB8^TF/ZH11 lines (k) at different time points of the day in June 2021 in Guangzhou. Values are means ± SEM. (n = 8 panicles). d, g, l Number of opened florets in ZH11 and Osmyb8^ZH mutants (d), TFB and Osmyb8^TF mutants (g), and ZH11 and OsMYB8^TF/ZH11 lines (l) at different days after heading in June 2021 in Guangzhou. Values are means ± SEM. (n = 10 panicles). h Schematic diagram of the vector structure used for constructing OsMYB8^TF/ZH11 transgenic plants. i RT-qPCR analysis of OsMYB8 transcripts levels in the lodicules of the OsMYB8^TF/ZH11 transgenic lines. ZH11 was used as a negative control. Values are means ± SEM. (n = 3 biological replicates). Significance is evaluated by the two-sided Student’s t-test, and P values are indicated. Source data are provided as a Source Data file.