Fig. 5: OsJAR1 influences JA-Ile content in lodicule to regulate rice DFOT.

a Creation of the Osjar1 mutants in ZH11 background using the CRISPR/Cas9 genome editing approach. The mutation site was indicated in red. b Comparison of panicles in ZH11 and Osjar1 mutants at 11:30 am in June 2022 in Guangzhou. Scale bars, 1 cm. c Number of opened florets per panicle in ZH11 and Osjar1 mutants at different time points of the day. Values are mean ± SEM. (n = 10 panicles). d, e JA-Ile content in lodicules at 10:00 am of ZH11, Osjar1, and Osmyb8^ZH (d) at 9:00 am of ZH11 and OsMYB8^TF/ZH11(e). Values are mean ± SEM. (n =  3 biological replicates). Significance is evaluated by the two-sided Student’s t-test, and P values are indicated. f Schematic diagram of the vector structure used for constructing OsJAR1^com materials. The pOsMYB8^TF means the promoter was amplified from TFB. g Relative expression level of OsJAR1 in the lodicules of ZH11, OsJAR1^com and Osmyb8^ZH. Values are means ± SEM. (n = 3 biological replicates). Letters above the bars indicate significant differences (P < 0.05), as evaluated by one-way ANOVA with Tukey’s multiple comparisons test. h, j Comparison of panicles in ZH11, OsJAR1^com and Osmyb8^ZH at 11:30 am in June 2022 in Guangzhou (h) and in ZH11, OsMYB8^TF/ZH11, OsMYB8^TF/Osjar1 and Osjar1 at 12:00 noon in October 2022 in Guangzhou (j). Scale bars, 1 cm. i Number of opened florets in ZH11, OsJAR1^com, and Osmyb8^ZH at different time points of the day in June 2022 in Guangzhou. Values are mean ± SEM. (n = 10 panicles). k Number of opened florets in ZH11, OsMYB8^TF/ZH11, OsMYB8^TF/Osjar1, and Osjar1 at different time points of the day in October 2022 in Guangzhou. Values are mean ± SEM. (n = 10 panicles). Source data are provided as a Source Data file.