Fig. 2: The biosynthetic pathways of tri-oxygenated and tetra-oxygenated taxoids and characterization of the taxoid 9α hydroxylase.
From: Biosynthesis of the highly oxygenated tetracyclic core skeleton of Taxol
a The biosynthetic pathways of tri-oxygenated taxoids. Reaction steps and new compounds characterized in this study were marked in blue. New formed groups in compounds were marked with red. b HPLC analysis of in vivo reaction for S. cerevisiae strain YCYP725A37 expressing CYP725A37 and TCPR using 5α,13α-diacetoxytaxa-4(20),11(12)-diene-10β-ol (10) as the substrate. Substrate was marked with compound number and product was marked with an asterisk. S. cerevisiae strain YTCPR expressing TCPR was used as the control. c Mass spectra (ESI) of new peak (*). These experiments were performed three times with similar results each time. d The C9 hydroxylation reaction catalyzed by CYP725A37 to produce 9,10-deacetyl taxusin (11) from 10. Source data are provided as a Source Data file.
