Fig. 2: Establishing a single nucleus atlas of mouse hypothalamus from postnatal day 15 (P15) male and female offspring raised by mothers fed with different dietary fat during lactation. | Nature Communications

Fig. 2: Establishing a single nucleus atlas of mouse hypothalamus from postnatal day 15 (P15) male and female offspring raised by mothers fed with different dietary fat during lactation.

From: Maternal dietary fat during lactation shapes single nucleus transcriptomic profile of postnatal offspring hypothalamus in a sexually dimorphic manner in mice

Fig. 2

a Diagram of single nucleus RNA-seq experiment using 10x Genomics (n = 2 for each maternal dietary group, groups including: fLHD female offspring under maternal low fat diet during lactation, fHFD, female offspring under maternal high fat diet during lactation, mLFD male offspring under maternal low fat diet during lactation, mHFD male offspring under maternal high fat diet during lactation). This image was adapted and created with BioRender.com. b Uniform Manifold Approximation and Projection (UMAP) visualization of key lineages after data integration, colored by different cell types (total cell number after QC = 38,594). c Canonical marker genes for key lineages shown on the UMAP. d Dotplots show the marker genes for key lineages. e Donut plots of the cell proportions of key lineages from female and male offspring under maternal LFD and HFD exposure.

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