Fig. 4: Heterogeneity of AgRP/Npy and Avp/Rorb neurons in P15 offspring hypothalamus. | Nature Communications

Fig. 4: Heterogeneity of AgRP/Npy and Avp/Rorb neurons in P15 offspring hypothalamus.

From: Maternal dietary fat during lactation shapes single nucleus transcriptomic profile of postnatal offspring hypothalamus in a sexually dimorphic manner in mice

Fig. 4

a UMAP of canonical marker genes for AgRP neurons (Agrp and Npy) in neuronal subpopulations. b UMAP of canonical marker genes for AgRP neurons (Agrp and Npy) on the extracted AgRP/Npy neurons. Unsupervised clustering of extracted AgRP/Npy subclusters identified three distinct subtypes. c Barplot of the cell proportions of AgRP subclusters from fHFD, fLFD, mHFD and mLFD groups. d Violin plots of the expression of Agrp, Xist, Brd1 and Hdac3 genes in four groups in AgRP/Npy neurons. * represents P < 0.05 for Wilcoxon test. e Unsupervised clustering of extracted Avp/Rorb subclusters identified two distinct subtypes. f Heatmap of the top 10 differentially expressed genes in each Avp/Rorb subcluster. g Umap and Violin plots of the expressions of Avp, Rorb, Igfbp5 and Gm42418 genes in four groups in Avp/Rorb neurons. * represents p < 0.05, ** represents p < 0.01 and *** represents p < 0.001 for Wilcoxon test. ns represents no significance between comparisons.

Back to article page