Fig. 5: Heterogeneity of astrocytes and oligodendrocytes in P15 offspring hypothalamus. | Nature Communications

Fig. 5: Heterogeneity of astrocytes and oligodendrocytes in P15 offspring hypothalamus.

From: Maternal dietary fat during lactation shapes single nucleus transcriptomic profile of postnatal offspring hypothalamus in a sexually dimorphic manner in mice

Fig. 5

a Unsupervised clustering of extracted astrocytes identified two distinct subtypes and the expression of canonical marker genes Gfap (Astrocytes-1) and Slc7a10 (Astrocytes-2) on UMAP. b Trajectory analysis of astrocytes identified a trajectory from subtype Astrocyte-1 to Astrocyte-2. Trajectory related gene expressions along the pseudotime including Myoc, Id1, Id3 and Id4 that represents a more-stemmed status were pseudo-gradually down-regulated wheares genes including Plpp3, Cst3 were up-regulated along the trajectory. c, d UMAP of unsupervised clustering of extracted oligodendrocytes identified five distinct subtypes and the expression of canonical marker genes Pdgfra (oligodendrocyte precursor cells) and Plp1 (oligodendrocytes). Trajectory analysis shows the trajectory from ODC-1 to ODC-5. e Violin plots of canonical marker genes Pdgfra and Plp1 in five oligodendrocyte subclusters. Plp1 shows a gradual change of expression along the differentiation trajectory.

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