Fig. 6: Cell-cell interactions in offspring hypothalamus revealed sex- and maternal diet-specific effects. | Nature Communications

Fig. 6: Cell-cell interactions in offspring hypothalamus revealed sex- and maternal diet-specific effects.

From: Maternal dietary fat during lactation shapes single nucleus transcriptomic profile of postnatal offspring hypothalamus in a sexually dimorphic manner in mice

Fig. 6

a Total numbers of cell-cell interactions in fHFD, fLFD, mHFD and mLFD groups. b Overall interactive signaling changes on key neuronal subpopulations and top scoring non-neuronal populations between mHFD and fHFD groups. c Immunohistochemistry staining shows the co-localization of AgRP/Npy neurons and astrocytes. Green cells marked by GFP represent AgRP/Npy neurons, purple cells marked by S100β represent astrocytes, blue dots marked by DAPI represent the nuclei. White overlapping by both GFP and S100β represents the colocalization of AgRP/Npy neurons and astrocytes. d Cell counts of colocalized AgRP/Npy neurons and astrocytes in four groups. Biologically independent animals for fHFD group were 5, for fLFD group were 5, for mHFD group were 5, for mLFD group were 4. p value by two-sided Student’s t test. Values are means ± s.d. * represents p < 0.05 for Wilcoxon test. e The identified key pathways of the interactive signaling between mHFD and fHFD groups. f, g The identified key up-regulated and down-regulated signaling ligand-receptor pairs between the interactions of AgRP neurons and astrocytes in mHFD and fHFD groups. Source data is provided as a Source Data file.

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