Fig. 2: TAD dynamics along p53 activation.

A Distribution of TAD sizes along p53 activation and inactivation. A two-sided Mann-Whitney test was used to test whether distributions of TAD sizes differed throughout p53 activation compared to control. Nut 0 h refers to control cells without p53 activation. The boxes show the interquartile range (IQR), the central line represents the median, the whiskers add 1.5 times the IQR to the 75 percentile (box upper limit) and subtract 1.5 times the IQR from the 25 percentile (box lower limit). Significance calculated with two-sided Mann–Whitney test. The star indicates p values below 0.01. Sample sizes of the box plots appear in the next panel. B Bar plot displaying the number of variant and invariant TAD borders along p53 activation and inactivation. Invariant TAD borders, in light gray, are those detected at all time points. Pie charts and positive/negative numbers represent the number of TAD borders gained/lost between the corresponding time points. C Principal Component (PC) Analysis of TADbit TAD border scores for all Hi-C biological replicates across p53 activation and inactivation. Principal components 1 and 2 were taken into consideration. Numbers in parentheses represent the percentage of variance explained by each principal component. D Ward hierarchical clustering of TAD borders based on their TAD insulation scores and the patterns of temporal changes observed throughout p53 activation and inactivation. Only TAD borders characterized by a TADbit score >4 were included. TAD borders were manually clustered considering their cluster robustness throughout p53 activation. Cluster C5 contains all TAD borders from clusters with low support. E Hi-C contact maps of a 6 Mb genomic region of chromosome 1 where TADs are marked by white lines. F Insulation score profiles stacked over a 1 Mb genomic region centered at TAD borders. Only TAD borders characterized by a TADbit score >4 were included. Sigmoid insulation score is inversely proportional to insulation capacity.