Fig. 3: CWC15 associates with the DCL1 complex.
From: The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis
a The interactions with CWC15 with DCL1, HYL1, SE and DDL analyzed by BiFC. Paired cYFP- and nYFP-fusion proteins were co-expressed in leaves of N. benthamiana. Green color indicates the BiFC signal detected by a confocal microscopy at 48 h after infiltration. Scale bar = 10 μm. Three independent experiments yielded consistent results. b Co-IP between MYC-CWC15 and HYL1−2HA. HYL1-2HA and GFP-2HA were co-expressed with MYC-CWC15 in leaves of N. benthamiana, respectively. IPs were performed using anti-MYC antibodies. HYL1-2HA, GFP-2HA and MYC-CWC15 were detected by western blot. Two independent experiments yielded consistent results. c Co-IP between MYC-CWC15 and SE-2H. SE-2HA and GFP-2HA were co-expressed with MYC-CWC15 in leaves of N. benthamiana, respectively. IPs were performed using anti-MYC antibodies. SE-2HA, GFP-2HA and MYC-CWC15 were detected by western blot. Two independent experiments yielded consistent results. d Co-IP between CWC15 and SE or HYL1 in pCWC15::GFP-CWC15/ amiRCWC15 transgenic plants. Co-IP was performed using anti-GFP antibodies. GFP-CWC15, SE and HYL1 were detected by western blot. Two independent experiments yielded consistent results. Source data are provided as a Source Data file.
