Fig. 3: GCR1/2 interact with SlTOE1B in vivo.
From: Spatiotemporal formation of glands in plants is modulated by MYB-like transcription factors
a Co-immunoprecipitation (CoIP) assay shows GCR1/2 and GCR1/2-C could interact with SlTOE1B in vivo. GCR1, N-terminal domain of GCR1 (GCR1-N), C-terminal domain of GCR1 (GCR1-C), GCR2, GCR2-N and GCR2-C were fused with GFP. SlTOE1B fused with FLAG. GFP (left lane, negative control) or GFP fused with the target protein (other lanes) were used as bait to bind to the GFP beads. Immunoprecipitated proteins were detected by anti-FLAG. b Results of bimolecular fluorescence complementation (BiFC) assays in N. benthamiana leaf epidermal cells show GCR1/2 interacted with SlTOE1B through its C-terminal domain. GCR1/2 and their fragments fused with C-terminal domain of YFP (nYFP). SlTOE1B fused with N-terminal domain of YFP (cYFP). Three biological repeats were performed for each interaction. Bar: 50 μm.
