Fig. 2: SNX8 binds to and tubulates lysosomes.
From: SNX8 enables lysosome reformation and reverses lysosomal storage disorder
A Representative images of WT, SNX2-KO, SNX8-KO, and SNX2/8-DKO HeLa cells stained with lysotracker for 30 min. Scale bar = 10 μm. B, C Quantifications of the total intracellular lysotracker intensity (B) and the size of largest lysosomes in cells (C) of cell groups shown in (A) (n = 5, 3, 4, 4, 3, 3, respectively, for each group for (B) and n = 25 for (C). For (B), p = 1 (WT vs. SNX2-KO), 0.027 (WT vs. SNX8-KO, <0.00001 (WT vs. SNX2/8-DKO), 0.28 (WT vs. SNX2/8-DKO + GFP-SNX8), 0.40 (SNX2/8-DKO vs. SNX2/8-DKO + GFP-SNX2), 0.00002 (SNX2/8-DKO vs. SNX2/8-DKO + GFP-SNX8). For (C), p = 0.25 (WT vs. SNX2-KO), <0.00001 (WT vs. SNX8-KO, 0 (WT vs. SNX2/8-DKO), 0.18 (WT vs. SNX2/8-DKO + GFP-SNX8), <0.00001 (SNX2/8-DKO vs. SNX2/8-DKO + GFP-SNX8)). D WT and SNX8-KO HeLa cells were immunoprecipitated against LAMP1, and blotted against LAMP1 and SNX8. E SFB-SNX8 or SFB-SNX8-K135A were purified from HEK 293T cells and subjected to PIP Strips with indicated lipid dots. Fraction 5 (biotin-eluted) was used for the assay as indicated with red rectangles. F Surface scanning EM images of negative-stained lysosomes purified from SNX8-KO cells with or without 20 min incubation with 10 μM of SNX8 or SNX8-K135A proteins. Scale bars = 250 nm (left) or 100 nm (right). For graphs, error bars are s.e.m, statistical comparison was done using one-way ANOVA, Tukey test. Source data are provided as a Source Data file.
