Fig. 3: Cryo-EM structure and functional analysis of BLV-bound NTCP.
From: Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP
a Normalized inhibition of TC transport (1 μM) into Flp-In T-Rex cells stably expressing NTCP at varying concentrations of BLV. Uptake of 3H-TC was measured after 8 min, each data point indicates the mean of three independent replicates, and error bars represent the SD. b Size exclusion chromatography and SDS-PAGE analysis of purified human NTCP in a complex with BLV, Fab3, and Nb. c Cryo-EM density map (left) and model in ribbon representation (right) of NTCP-BLV-Fab3-Nb complex. The BLV peptide is colored in three sections: myristoylated glycine (magenta), plug (orange), and string (blue). The densities and models are colored as follows: NTCP in green, Nb in gray and Fab3 in purple (heavy chain) and coral (light chain).
