Fig. 5: Cerebral vessels imaging with high spatial resolution and stability in the NIR-III region. | Nature Communications

Fig. 5: Cerebral vessels imaging with high spatial resolution and stability in the NIR-III region.

From: Lanthanide luminescence nanothermometer with working wavelength beyond 1500 nm for cerebrovascular temperature imaging in vivo

Fig. 5

a Time-sequence cerebral vessels imaging of control and hypothermia mice under an excitation of 808 nm laser at 120 mW cm−2 with 1500 nm long pass filter and an exposure time of 300 ms. b Time-dependent quantitative cerebral vessel luminescence intensities (red dash square) of control mouse and hypothermia mouse. The intensities were normalized at 0 s to compare the changing behavior of the two groups. Data were presented as mean values based on three continuous images (n = 3). Error bars were defined as standard deviation. c Representative cerebral vessels image under the same condition as (a). The vessels including inferior cerebral veins (ICV), superior sagittal sinus (SSS), superficial veins (SV), and the transverse sinus (TS) were labeled in red arrows. d Plot profile data (black curve) recorded along the red dash lines in (c). Gaussian fittings (red curves) of the peaks in the profile provided the full width at half maximum (FWHM) values of ICV, SSS, SV, and TS in NIR-III imaging.

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