Fig. 6: Identification of MT inner surface binding GFG repeats in MIPs. | Nature Communications

Fig. 6: Identification of MT inner surface binding GFG repeats in MIPs.

From: Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function

Fig. 6

a Multiple sequence alignment of the GFG repeat in two human and two C. reinhardtii proteins identified by iterative HMM-to-HMM searches. Proteins are designated by their UniProt identifiers. Coloring schemes as per ClustalW parameters with modifications. b Module architecture and evolutionary relationships of the GFG repeat family members in C. reinhardtii and humans. c The GFG repeat 2 in human EFHB. d Module architecture of C. reinhardtii FAP21 (human EFHB) showing the GFG repeats along the length of FAP21. In FAP21, four GFG repeats were identified by sequence alignment and a fifth was inferred from visual inspection of the FAP21 tertiary structure in the C. reinhardtii outer doublet. e Superimposition of four GFG repeats from FAP21. The highly divergent central portion of each GFG repeat (dotted line) is omitted to allow superimposition. f One GFG repeat unit of FAP21 in contact with the MT lattice. Contact sites are highlighted in red. Conserved aromatic and glycine residues are shown (g) One GFG repeat unit of EFHB in contact with the MT lattice. Contact sites are highlighted in red. Conserved aromatic and glycine residues are shown.

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