Fig. 8: The ELLEn module binds FAP67 and NME7 in the cilia outer doublet lumen.

a Multiple sequence alignment of the ELLEn module across species. Proteins are designated by their UniProt identifiers. Species represented are Homo sapiens (Hs), Mus musculus (Mm), Xenopus tropicalis (Xt), Danio rerio (Dr), Drosophila melanogaster (Dm), and C. reinhardtii (Cr). Coloring schemes as per ClustalW parameters with modifications. b Module architecture and evolutionary relationships of the ELLEn module family members across species. Phylogenetic relationships are calculated using average distances and percent identity (PID) between ELLEn modules used in the alignment. c Associations of the ELLEn module in FAP53 with FAP67 (NME7 in human). d, e AlphaFold2 prediction of the human complex and the contacts between the ELLEn module of TCHP and NME7. f Schematic representations of FLAG-NME7 constructs. g FLAG pulldown analysis and immunoblot of FLAG-NME7 constructs expressed in HEK293T cells. Proteins were probed with the indicated antibodies. The immunoblots are representative of two independent experiments. h Schematic representations of FLAG-TCHP constructs. i FLAG pulldown analysis and immunoblot of FLAG-TCHP constructs expressed in HEK293T cells. Proteins were probed with the indicated antibodies. The immunoblots are representative of three independent experiments. j Quantitative analysis of centrosome-associated proteins during centriole biogenesis. An array of representative centrosome proteins implicated in centriole duplication are shown in solid lines indicating temporal dynamics of centrosome protein recruitments. NME7 and TCHP are indicated with dashed lines. The quantitative analysis is representative of experiments performed in two replicates. Source data are provided as a Source Data file.