Fig. 3: Comprehensive analysis of three tissues in the initiation phase and the discovery of the involvement of the IL-33/ST2 axis.
From: The IL-33/ST2 axis is protective against acute inflammation during the course of periodontitis
a KEGG pathway enrichment analysis based on the upregulated DEGs in the GT and PRT using GO/KEGG enrichment analysis tools based on clusterProfiler in Hiplot (Benjamini & Hochberg-method adjusted P value < 0.05; false discovery rate < 0.1; fold-change of normalized counts ≥ 2). The top 20 pathways are shown with their expression counts and adjusted (Benjamini & Hochberg method) P value. b Volcano plots of DEGs in the GT and PRT. The top 5 genes with the highest adjusted P value were annotated. DEG differentially expressed gene. c, e, g mRNA expression level of Il1rl1 (c), Il1rl1 variant 1 (e), and Il33 (g) on the control side of three tissues (on Day 1; GT = 1; n = 4 mice per group except for the groups highlighted in Source Data file, which were n = 3 mice per group). d, f, h Temporal change in the mRNA expression of Il1rl1 (d), Il1rl1 variant 1 (f), and Il33 (h) in the three tissues (control Day 1 of each tissue = 1; n = 4 mice per group except for the groups highlighted in Source Data file, which were n = 3 mice per group). GT gingival tissue, PRT peri-root tissue, BT bone tissue, D1 Day 1, D3 Day 3, D5 Day 5, D8 Day 8, D14 Day 14. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns (not significant), P > 0.05; by one-way ANOVA with multiple comparisons via Tukey’s test (c, e, g); and two-way ANOVA with multiple comparisons via Šídák’s method (d, f, h). The obvious outliers were evaluated and excluded by Grubb’s test (α = 0.05). Exact P values are presented in Supplementary Data. 1. Source data are provided as a Source Data file.
