Fig. 2: Cryo-EM structure of full-length ERGIC-53 in complex with MCFD2.
From: Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport
a Representative 2D class-average image of the full-length particles. The upper and lower panels represent straight and bent particles, respectively. b Cryo-EM map of full-length ERGIC-53 in complex with MCFD2. c The overall architecture of full-length ERGIC-53 overlaid in the EM map shown in (b). d, e Results of 3DVA of the full-length ERGIC-53 with two variability components I (d) and II (e). The 3D EM maps of the first (pink) and last frames (blue) of continuous conformational changes generated by 3DVA are displayed. Broken lines represent the center of three segments. Red circles represent hinges between the segments 1, 2, and 3. The lower panels show the top views of the two maps superimposed on each other. The brown broken line represents the interface-H between the upper CRDs.
