Fig. 4: Role of the long stalk and the head assembly in cargo transport.
From: Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport
a Schematic representation of the ERGIC-53 mutants used for the rescue experiments. b, c Rescue experiments of FV-HiBiT (b) and AAT-HiBiT (c) secretion by coexpression of ERGIC-53 or its mutants in ERGIC-53 KO cells. The amounts of secreted FV/AAT relative to those of intracellular FV/AAT were quantified with a commercially available HiBiT-tagged protein detection reagent and normalized to those from the KO cells transfected with an empty vector. Each symbol represents the individual data points. Error represents the standard deviation of the mean (N = 3 biological replicates). Statistical significance was measured with one-way ANOVA followed by Dunnett’s test. Exact P values are indicated in the graphs for each construct. d A working model of cargo capture by ERGIC-53.
