Fig. 1: Generating hybrid and gene expression profiles from two divergent Ciona species. | Nature Communications

Fig. 1: Generating hybrid and gene expression profiles from two divergent Ciona species.

From: Temporospatial hierarchy and allele-specific expression of zygotic genome activation revealed by distant interspecific urochordate hybrids

Fig. 1

a Morphology of adult C. robusta (left) and C. savignyi (right). Scale bar: 1 cm. b Phylogenetic position and divergence time estimation of Ciona. Divergence times are displayed below the phylogenetic tree. c Different developmental stages of four cross ascidians, including self-crossing C. robusta, self-crossing C. savignyi, C. savignyi as a male parent cross with C. robusta (forward cross), and C. robusta as a male parent cross with C. savignyi (reverse cross). Eight developmental stages were observed including 2-, 4-, 8-, 16-, 32-, and 64-cell, early-neurula (earN), and tailbud stages. Scale bar: 50 μm. The cross experiment was independently performed at least three times. d Overview of the gene expression profiling strategy. Forward and reverse cross embryos collected for bulk RNA sequencing included 1-, 2-, 4-, 8-, 16-, 32-, 64-, and 112-cell, mid-gastrula (midG), earN, and late-neurula (lateN) stages. Cross embryos at the 64- and 112-cell and earN stages were collected for single-cell RNA sequencing.

Back to article page