Fig. 7: Evoked bursts self-reinforce the diversity of burst activity in a CaM kinase-dependent manner. | Nature Communications

Fig. 7: Evoked bursts self-reinforce the diversity of burst activity in a CaM kinase-dependent manner.

From: Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons

Fig. 7

A Representative image of sorted bursts evoked by optogenetic stimulation. A total of 891 burst traces are presented. Light stimulation induced evoked spikes, and the burst responses persisted after light stimulation. Secondary and tertiary responses were also observed. B Latency of the evoked bursts. Repeated light stimulation (at second and third attempts) significantly decreased evoked burst latency in control and anisomycin-treated conditions, whereas K252a treatment did not influence the latency. n = 3 organoids. P = 0.0179; 0.0033 (2nd and 3rd, in comparison to 1st within control). C (i) Overlayed power histograms of evoked bursts and kernel density estimation (line) in the presence of K252a and anisomycin. Repeated light stimulation increased burst response complexity. (ii) Percentage of evoked bursts with and without secondary peaks. D Violin plots of burst response peak times. Red line indicates median. n = 50 bursts. E Representative crosstalk between the two connected organoids in self-evoked bursts. Color-coded maps (bottom circles) depict the voltage distribution from neurons in left and right cerebral organoids. *p < 0.05; one-way ANOVA followed by Tukey’s multiple-comparison test. Data are presented as mean values ± SD.

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