Fig. 1: Experimental overview of autologous ENSC transplantation.
From: Autologous cell transplantation for treatment of colorectal aganglionosis in mice
A Timeline of experiments for isolating and expanding intestinal ENSCs from adult Wnt1-iDTR mice followed by autologous transplantation to diphtheria toxin-induced aganglionic colon. Immunohistochemical and functional assays were performed 1–8 weeks following cell transplantation. B End-to-end small intestinal anastomosis was performed (arrows) following segmental bowel resection. C Histologic examination confirmed tissue repair at the anastomosis one week after surgery. D ENSCs were expanded as neurospheres and labelled with lentiviral-GFP (D’). E Scatter plot and corresponding regression line show a linear relationship between length of bowel resected and number of neurospheres generated. DT,diphtheria toxin; EFS,electrical field stimulation. Scale bars 100 µm (C, D′), 200 µm (D).
