Fig. 3: Replication and transmission of sylvatic dengue virus serotype 2 (DENV-2) is similar in native (cynomolgus macaque) and novel (squirrel monkey) hosts.

Sylvatic dengue virus serotype 2 (DENV-2) replicates to low levels and transmits rarely to Ae. albopictus in infected: (A) cynomolgus macaques and (B) squirrel monkeys. Points for mosquito infections that occurred from monkeys without detectable viremia are placed at arbitrary positions below the limit of detection (LOD) for visualization only. Viremia was monitored by direct titration of serum as well as one passage of serum followed by titration; transmission was monitored by feeding cartons of 10-15 uninfected mosquitoes on each monkey; note that usually fewer than the full complement of mosquitoes engorged. Source data are provided as a Source Data file. Images used under license from Shutterstock.com.