Fig. 5: Replication and transmission of sylvatic Zika virus (ZIKV) is similar in native (cynomologus macaque) and novel (squirrel monkey) hosts.

ZIKV replicates robustly and transmits efficiently to Ae. albopictus in (A) cynomolgus macaques (n = 3 biologically independent animals) and (B) squirrel monkeys (N = 10 biologically independent animals) from a single independent experiment each. Control animals are not included in this figure. Blue lines indicate mean viremia, bands indicate ± 1 standard error (SE), and black dots indicate viremia for each individual monkey. Bars indicate mean % mosquitoes infected per monkey, and error bars indicate ± 1 standard error (SE). In panel A, the inset shows the dynamics of viremia and transmission during the window of viremia for each individual macaque. Viremia was monitored by direct titration of serum as well as one passage of serum followed by titration; transmission was monitored by feeding cartons of 15 uninfected mosquitoes on each monkey; note that usually fewer than 15 engorged. Source data are provided as a Source Data file. Images used under license from Shutterstock.com.