Fig. 6: Novel hosts transmitted ZIKV to mosquitoes more efficiently than native hosts.

A Relationship between virus titer in serum and transmission of sylvatic Zika virus (ZIKV) to legs of Ae. albopictus from cynomolgus macaques (light blue) or squirrel monkeys (dark blue). Points show raw data, with point size proportional to the number of mosquitoes tested in the batch (one day in one monkey). B–E Relationships between virus titer in serum and transmission of ZIKV to legs of Ae. albopictus from both non-human primate species combined (blue curve, repeated in B–E) or transmission to legs of Ae. aegypti from humans of (B) Dengue virus (DENV) serotype 1, (C, D) DENV serotype 2, (E) DENV serotype 4. DENV curves were fitted to data from Duong et al.⁵¹ for sub-panels (B, C, E), and to data from Long et al. 2019⁵² for sub-panel (D). Infectious dose 50 (ID₅₀) and confidence interval (CI) provided for each designated virus and mosquito species in log₁₀ pfu/ml. Model selection was conducted with three competing functional forms, all with a sigmoidal shape. Curves represent the best fit, and shaded areas correspond to 95% confidence intervals of the fitting process. ^a CI of ID₅₀ not provided as it was too wide to be insightful. ^b ID₅₀ and CI not provided as they were not insightful. Note that data used for fitting of this DENV-2 curve was from mosquito heads rather than legs, both are markers of a disseminated infection. Source data are provided as a Source Data file.