Fig. 2: i53 variants display additional molecular interactions with 53BP1. | Nature Communications

Fig. 2: i53 variants display additional molecular interactions with 53BP1.

From: Functional screening in human HSPCs identifies optimized protein-based enhancers of Homology Directed Repair

Fig. 2

Crystal structures of i53 variants bound to 53BP1 Tudor domain. A Structural alignments of 53BP1 Tudor domain (yellow) bound to i53 variants: WT (blue, PDB: 8SVG), L67H (light green, PDB: 8SVI), L67R (dark green, PDB: 8SVH), T12V.T14H.L67H (light red, PDB: 8SVJ), and T12Y.T14E.L67R (dark red, PDB: 8T2D). B Zoomed in view of the complex at the solvent-exposed loop preceding β5 for i53, L67H, and L67R. C Zoomed in view of the complex near i53 residues 12 and 14 for wild-type i53, T12V.T14H.L67H, and T12Y.T14E.L67R. Hydrogen bonds are denoted by black dotted lines.

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