Fig. 2: Histamine recognition and binding of H4R.
From: Structural basis of ligand recognition and design of antihistamines targeting histamine H4 receptor
a The ligand binding pocket of histamine. b A schematic map of histamine/receptor interaction. Green color, hydrophobic interaction; purple color, polar interaction. c Conservation of key residues of the ligand binding pocket among histamine receptor family. d A comparison of histamine binding between H4R and H1R (PDB:7dfl). e [3H] histamine and [3H] JNJ7777120 binding for H4R mutants. f pKi of histamine binding of H4R mutants. g pEC50 of Gi activation of H4R mutants by histamine. From e to g, data are presented as mean values ± SEM.; n = 4–9 independent experiments for e, n = 3–4 independent experiments for f, and n = 3–4 independent experiments for g. Each point in the figure represents an independent experiment. Source data are provided as a Source Data file.
