Fig. 2: The pericloacal mesenchyme of the Tgfbr1-cKO embryos adopts hindlimb fate. | Nature Communications

Fig. 2: The pericloacal mesenchyme of the Tgfbr1-cKO embryos adopts hindlimb fate.

From: Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscape

Fig. 2

Ventral views of the hindlimb/cloacal region of wild type (A–H) or Tgfbr1-cKO embryos (A’–H’) stained for Fgf8 (E10.5)(A, A’), Lin28a (E10.5) (B, B’), Pitx1 (E10.5) (n = 2); C, C’), Fgf10 (E10.5) (D, D’), Wnt5a (E11.0) (E, E’), Hand2 (E11.0) (F, F’), Tbx5 (E11.5) (G, G’) or Isl1 (E11.5) (H, H’). Fgf8 expression shows posterior and medial extension of the AER (black arrow) and the absence of expression in the cloacal endoderm (black arrowhead). Hindlimb markers Lin28a, Pitx1 and Fgf10 extend into the pericloacal region Tgfbr1-cKO embryos (white arrows). Wnt5a and Hand2 are expressed in the posterior prominence of the Tgfbr1-cKO hindlimbs, while forming clear separation of the hindlimb and GT domains in the control embryos (yellow arrowheads). Pericloacal expression of Wnt5a, Tbx5 and Isl1 is lost in the mutant embryos (yellow arrows). Isl1 expression in cloacal endoderm is marked by white arrowhead. At least n = 3 embryos were analyzed per probe, unless stated otherwise, giving equivalent patterns.

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