Fig. 1: Phylogenetic and bioinformatic analysis of enterobacterial DyP encapsulins and heterologous production of KpDyP_Enc.

a Representative phylogenetic tree of DyP encapsulins found in the Enterobacteriaceae family. Genera with prominent pathogenic members are shown in bold. The genus of interest for this study (Klebsiella) is shown in red. The inset highlights the number of distinct DyP encapsulins identified in the listed enterobacterial genera. b Representative K. pneumoniae DyP encapsulin operon. The generally four conserved genes of the operon are flanked by various types of transposases (Tnp, gray). FDH NAD-dependent formate dehydrogenase (light blue), DyP dye-decolorizing peroxidase cargo (red; targeting peptide (TP): orange), Enc Family 1 encapsulin shell protein (blue), RpoH RNA polymerase sigma factor (turquoise). Dashed outlines indicated mostly but not strictly conserved genes. kb kilobase. c Sequence logo for TPs found in DyP cargos highlighting the conserved targeting motif. n number of cargo sequences used. d Size exclusion chromatogram of KpDyP_Enc (Superose 6). The elution volume (12 mL) of the labeled main peak is consistent with a T1 encapsulin while the co-eluting 410 nm heme signal suggests DyP encapsulation. e SDS-PAGE analysis of purified KpDyP_Enc. This purification was repeated independently six times. Source data are provided as a Source Data file. f Negative stain transmission electron micrograph of purified KpDyP_Enc. This experiment was repeated independently five times. Scale bar: 50 nm.