Fig. 6: Comparative transcriptomic analysis of 8-cell embryos from WT, atg5-2 mutant, and atg5-2 mutant complemented with integumentary tapetum-specific ATG5 expression.
From: Autophagy-mediated degradation of integumentary tapetum is critical for embryo pattern formation
a Principal component analysis of transcriptomes of 8-cell embryos from the WT, atg5-2 mutant, and atg5-2 mutant expressing proTPE8:ATG5-GFP. Three biological replicates for each genotype (n = 3). b Heat map showing Pearson’s correlation across RNA-seq datasets. c, d Volcano plot showing differentially expressed genes between atg5-2 and WT embryos (c) or between atg5-2 and atg5-2 mutant expressing proTPE8:ATG5-GFP embryos (d). Red dots indicate upregulated genes, while blue dots indicate downregulated genes. The Benjamini–Hochberg method was used to adjust the two-sided P values for multiple comparisons. FC fold change, Adjusted P, Benjamini–Hochberg adjusted P value. e Comparisons of differential genes between atg5-2/WT and atg5-2/atg5-2 proTPE8:ATG5-GFP. f Differentially expressed genes between atg5-2 and WT could be categorized into four distinct clusters. DEGs located in clusters I and III are regulated by ATG5 in the integumentary tapetum, whereas DEGs in clusters II and IV are not. Gene ontology enrichment analysis of genes in four different groups. FDR false-discovery rate.
