Fig. 8: Altered triacylglycerol (TG) levels in the integumentary tapetum of autophagy-deficient mutant.
From: Autophagy-mediated degradation of integumentary tapetum is critical for embryo pattern formation
a–c Lipid droplets in the integumentary tapetum from the WT (a), atg5-2 (b), and atg7-1 (c) mutants were stained with BODIPY 493/503. Arrows indicate lipid droplets. IT integumentary tapetum, DAP days after pollination, SR2200 SCRI Renaissance 2200. Scale bars: 20 µm (left); 5 µm (right). d, e Quantitative analysis of the area (d) and number (e) of lipid droplets within the integumentary tapetal cells of WT, atg5-2, and atg7-1 mutants. The data are displayed in the box and whisker plots. Center line, median values; top and bottom of boxes, 75th and 25th percentiles; top and bottom lines, maximum and minimum values (n = 20 independent samples), (two-tailed Student’s t test, ****P < 0.0001).
