Fig. 5: Proximity labeling with TurboID identified proteins interacting with OsPUX8B.2 in rice.

a Workflow of proximity-labeling spectrometry (PL-MS/MS) using rice seedlings harboring the indicated TurboID-fusion constructs. b–e Ratiometric analysis of OsPUX8B.2 preys identified by PL-MS/MS. The protein enrichment areas (LFQ intensities) were used for all quantitative ratiometric analyses. The cutoffs of adjusted p-value < 0.05 and Log₂(FC) > 1 were used for selection of putative preys of OsPUX8B.2. In b, non-MG132-treated samples were analyzed by comparing biotin-treated (+biotin) data with non-biotin-treated (−biotin) data. In c–e, all seedlings were treated with MG132, and the MS data were quantified by comparing the OsPUX8B.2+biotin data with the OsPUX8B.2−biotin (c), GFP+biotin (d), and GFP−biotin (e) data. f Gene Ontology (GO) term enrichment analysis of proteins present in at least two groups from the quantitative analysis in c–e. g Venn diagram showing the extent of overlap between proteins identified in c–e. The 58 core preys (CPs) are labeled in yellow. h Relative protein abundance of the 58 CPs. Relative protein levels (Log₂LFQ) in MS data from three biological replicates were used for the analysis. Data were normalized per row for each protein using TBtools⁷⁶. The protein OsBHT (encoded by LOC_Os01g55270.1) is labeled in red. i Predicted subcellular localizations of the 58 CPs.