Fig. 3: Interactions between the Bre1 complex and the nucleosome.
From: Structure of the human Bre1 complex bound to the nucleosome
a Structure of Model I. The magnified regions in (b, c) are indicated. b Interactions between RINGA and the acidic patch in Model I. Hydrophilic interactions (salt bridges and hydrogen bonds) are indicated by gray dotted lines. c Basic residues of RINGB near the DNA phosphates in Model I. d A schematic representation of the protein constructs used in the experiment shown in (e). TS, Twin-Strep-tag. e H2BK120 ubiquitination assay of the wild type (WT) and mutants possessing substitutions at basic residues. The signals were normalized usig the normalization control. The mean and standard deviation of six (for WT) or three (for mutants) independent results are shown. Source data are provided as a Source Data file.
