Fig. 5: ATGL variant activity in live cells.
From: Mutational scanning pinpoints distinct binding sites of key ATGL regulators in lipolysis
A Confocal images of HeLa cells transfected with YFP-ATGL variants. Pictures are merged fluorescent images with Hoechst in cyan, YFP-ATGL in yellow, and Bodipy in magenta. Experiments were repeated independently three times with similar results. Transfected cell with LDs are counted in (B) with n > 393. B Fraction [%] of YFP-positive cells with LDs (magenta) and without LDs (yellow). Cells expressing YFP-ATGL from three independent experiments (black open dots) were classified according to presence of absence (<2) of LDs. n = total number of cells counted. Single amino acid ATGL variants are color-coded according to the most specific binding alteration with a single partner, color code: CGI-58 green, G0S2 yellow, PLIN1 cyan, and PLIN5 blue. Source data are provided as a Source Data file. C Confocal image of HeLa cells transfected with YFP-ATGL-F348E + R351L double mutant. Picture as in (A). Experiments were repeated independently three times with similar results.
