Fig. 2: Influence of mexY and oprM in the expression levels of lecA and pqs operon of mexZ* mutant. | Nature Communications

Fig. 2: Influence of mexY and oprM in the expression levels of lecA and pqs operon of mexZ* mutant.

From: Mutations in the efflux pump regulator MexZ shift tissue colonization by Pseudomonas aeruginosa to a state of antibiotic tolerance

Fig. 2

Fold changes of mexZ* (green), mexZ*ΔmexY (light green), mexZ* overexpressing (OE) oprM and its control with empty pSeva234 plasmid (light red and dark red, respectively) and ΔmexB (purple) were estimated regarding the expression of the PAO1 wild-type strain by RT-qPCR. Data are presented as mean values ± SEM of the results from three biological replicates with three technical replicates each. Statistically significant differences regarding PAO1 were calculated with two-sided t-test for paired samples assuming equal variances and indicated as ** (p < 0.005), *** (p < 0.0005) and **** (p < 0.00005): lecA expression in mexZ* (p = 0.00012), lecA expression in mexZ*ΔmexY (p = 0.4), lecA expression in mexZ*pSEVA234 (p = 0.000056), lecA expression in mexZ*oprM OE (p = 0.098), lecA expression in ΔmexB (p = 0.00038); pqsD expression in mexZ* (p = 0.0011), pqsD expression in mexZ*ΔmexY (p = 0.34), pqsD expression in mexZ*pSEVA234 (p = 0.000017), pqsD expression in mexZ*oprM OE (p = 0.054), pqsD expression in ΔmexB (p = 0.000002). Source data are provided as a Source Data file.

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