Fig. 9: SLAMR is implicated in contextual fear memory consolidation. | Nature Communications

Fig. 9: SLAMR is implicated in contextual fear memory consolidation.

From: Synaptically-targeted long non-coding RNA SLAMR promotes structural plasticity by increasing translation and CaMKII activity

Fig. 9

A For the acquisition experiment, mice were implanted with bilateral cannulas in the dorsal CA1 and then divided into three groups (sham, NC, and SLAMR_Gapmer) and infused with their corresponding agent 72hrs before the CFC training. B, C 1 hr after training these mice were placed again in the same context for a 5 min test. There were no differences between groups in the early retention test. (Sham n = 10, Control n = 14, SLAMR n = 13; data are presented as mean ± SEM). D SLAMR was KD in dorsal CA1 of mice implanted with bilateral cannulas 72hrs before the training and tested 24 hrs later in the same context. E, F The long-term memory test was carried-out 24h after the training and shows a significant reduction in the percentage of freezing time in SLAMR-KD compared to controls (Sham n = 10, Control n = 10, SLAMR n = 12; (E) Two-way ANOVA + Uncorrected Fisher’s LSD (*control vs SLAMR: min 1 p = 0.034, min 2 p = 0.0195, min 3 p = 0,0183, min 5 p = 0.0401; #Sham vs SLAMR: min 4 p = 0.0391, min 5 p = 0.0125) and (F) Two-way ANOVA + Tukey’s test; control vs. SLAMR **p = 6.88E-06, sham vs. SLAMR **p = 2.47E-04) Data as MEAN ± SEM. G Experimental design where SLAMR was KD after memory consolidation. H SLAMR KD 24hrs after finishing the training does not affect the expression of fear during the long-term memory test (Sham n = 10, Control n = 14, SLAMR n = 15; data as mean ± SEM). I The percentage of freezing during the 30 mins of the extinction process does not show any differences between groups (Sham n = 10, Control n = 14, SLAMR n = 15; data as mean ± SEM). J, K The Recall test was performed 24 hrs after the extinction shows an effective reduction in the expression of freezing in all the groups (Sham n = 10, Control n = 14, SLAMR n = 15; data as mean ± SEM). L Experimental design for SLAMR manipulation in CA3. M Plots show a similar percentage of freezing expression between groups during the training as well as (N), (O) 24h after the training the long-term memory test was performed (Sham n = 10, Control n = 11, SLAMR n = 12; data as mean ± SEM).

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