Fig. 1: Structure of SD1 and generation of anti-SD1 mAbs.
From: The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86
a Linear map of S marking S1 and S2 and showing elements of SD1 flanking RBD at both ends. b Structure of the S trimer showing positions of SD1, SD2, NTD, and RBD in S1. c Close up of structure of SD1 showing the N-terminal loop 322- 334 in cyan and the C-terminal fragment 527–591 in light green, the position of an intrachain disulfide bond is shown in yellow. d FACS sorting strategy used to isolate SD1 reactive memory B cells. e Neutralization potential of anti-SD1 mAbs. Non-neutralizing mAb (IC50 >10 μg/ml) do not achieve >50% neutralizing activity against Victoria and XBB.1.5 live virus at a concentration of 10 μg/ml.
